DEVELOPMENT OF METHOD OF ESTIMATION EFFICIENCY OF DECONTAMINATION OF PHOSPHORORGANIC COMPOUNDS

Abstract

Development of a method for evaluating the efficiency of decontamination in terms of the integral effects of the splitting of toxic substances on the human body. Paraoxon  is  the  model  substance  of  the  characteristic  chemical  structure  and toxicological characteristics. As a model system for determining the toxicity of paraoxone and its alkaline hydrolysis  products, the  inhibition  of  human  serum  butyrylcholinesterase  was  used.  Determination  of butyrylcholinesterase  activity  was  performed  ex  vivo  spectrophotometrically  using  the  modified  Ellman
method. It has been found that products of alkaline hydrolysis of paraoxone are almost 85 times less effective butyrylcholinesterase inhibitors of human serum than paraoxon. It was shown that the analysis of kinetics of inhibition of human blood serum butyrylcholinesterase in ex vivo conditions can be considered a  promising  quantitative  method  for  assessing  the  effectiveness  of  the  decontamination  method  of  toxic organophosphorus  compounds  from  the  point  of  view  of  the  effects  on  the  human  organism  of  cleavage products.
Originality.  For  the first time, the  method  of complex  quantitative  assessment of the  efficiency  of decontamination systems of organophosphorus compaunds from the point of view of the integral effects of the splitting of toxic substances on the human body has been proposed. Practical  value.  Based  on  the  evaluation  of  the  effect  of  phosphorus  organic  compounds  on  the inhibition  of  butyrylcholinesterase  in  humn  blood  serum,  the  most  effective  method  for  chemical decontamination  of  toxic  substances  can  be  selected,  objectively  evaluated  approaches  and  degassing systems  at  the  development  stage,  methods  for  correcting  the  composition  of  detergent  decontamination agents for technological equipment in chemical and pharmaceutical industries.